Research Article

Methyl Jasmonate Ameliorates Liver Oxidative Stress Associated with Acetic Acid-Induced Ulcerative Colitis in Wistar Rats

Abstract

Ulcerative colitis is the most common type of inflammatory bowel disease, with many extra manifestations. Reports have shown that over 30% of IBD patients presents with various liver disease. This study was carried out to investigate the effects of Methyl jasmonate (MEJ) in the reversal of liver oxidative stress associated with ulcerative colitis in a rat-model of ulcerative colitis. Fifteen male Wistar rats were used for this study and divided into 3 groups (n=5). Groups 1 (Control) and 2 (Colitis) were administered 1% ethanol three days before colitis induction. While, group 3 (MEJ + COL) received MEJ dissolved in 1% ethanol three days prior to colitis induction. Colitis was induced by administration of 2ml 4% acetic acid (v/v) intrarectally. The liver was collected six days post induction and protein concentration, myeloperoxidase, catalase and superoxide dismutase were determined by spectrophotometry. Protein concentration and myeloperoxidase levels rises significantly while, catalase and superoxide dismutase activities decreased significantly following colitis induction. Methyl jasmonate significantly decrease myeloperoxidase and increase catalase and superoxide dismutase activities in the liver compared to colitis group. Thus, in this study Methyl jasmonate was proven effective in reversing the oxidative stress in liver associated with ulcerative colitis and could be considered as an effective therapeutic agent in the treatment of inflammatory bowel diseases extra manifestations.